This can be shown by the following equation:2 H2O2 -------------------- 2 H2O + 2 O2This is a balanced equation with H2O being water and O2 being oxygen gas. You can identify abbreviated Project Ideas by the asterisk at the end of the title. Introduction In addition to your textbook, below are some web resourcesthat will add more background information about enzymes: Ineach individual cell of a human there are many chemical reactionstaking place, performing the necessary functions for being a large,complex, multicellular organism. What is happening in your test tube? You will be examining some ofthe major factors, which influence the activity of an enzyme calledcatalase. In order for an enzyme to perform its given job, it needs what is known as a substrate to bind to the active site of the enzyme so that the enzyme can speed up the reaction of the substrate.
The job of catalase in this experiment was to accelerate the breakdown of hydrogen peroxide into water and oxygen gas. Blend on high speed, pulsing when necessary, until the liver is smooth and no chunks are present. Adjust the height of the clamp so the open end of thecylinder is about 2 cm below the surface of the water. Figure 1 shows a pictureof the setup. The enzyme catalase, found in potato juice, was used for the catalyst along with a substrate known as hydrogen peroxide H2O2.
Catecholase is a reaction between oxygen and catechol… 3155 Words 13 Pages The Effect of Substrate Concentration on the Rate of Activity of the Enzyme Catalase Background Information: Enzymes are biological catalysts that increase the speed of chemical reactions without undergoing any physical change. Catalase The structure and function of enzymes is a central theme in cellular and molecular biology. In this lab, you will perform reactions involved in digestion of carbohydrates, lipids, and proteins and observe the results of these reactions. And because different body tissues have distinct environments—acidic or warm—each enzyme is tuned to work best under specific conditions. The bubbling reaction you see is the metabolic process of decomposition, described earlier.
For each parts A-F, you will begraphing enzyme activity on the Y-axis the dependent variable andthe independent variable parts A-F on the X-axis. In the food industry, it is used in combination with other enzymes in the preservation of foodstuffs and in the manufacture of beverages and certain food items. Using a potato and hydrogen peroxide, we can observe how enzymes like catalase work to perform decomposition, or the breaking down, of other substances. Construct an apparatus that allows you to collect and measure the oxygen gas produced for a description, see:. If you had to rely onthese reactions to take place spontaneously, you would wait a verylong time -- you wouldn't be here!. Pearson, as an active contributor to the biology learning community, is pleased to provide free access to the Classic edition of The Biology Place to all educators and their students.
Commercial and industrial designers create the shape and form of every type of manufactured good that you can think of—from toys, sporting goods, and medical equipment to high technology products, furniture, toothbrushes, and toasters. Graph your results as copper sulfate concentration M x-axis vs. This reaction is caused by catalase, an enzyme within the potato. Different tissues in the body have different pHs pH is a measure of how basic or acidic a solution is. The sample in test tube 2 reacted with the hydrogen peroxide and produced oxygen bubbles at a somewhat steady pace, while the sample in test tube 3 reacted vigorously at first and then slowed down as most of the enzyme had been used up. Keep the side with the disks upward andcarefully place the reaction vessel on its side in the pan of water. The main weakness of the investigation was the method.
But how does it do it? Catalase, an that brings about catalyzes the reaction by which is decomposed to and. Continue to hold the disk with the forceps. As pH comes closer to a neutral pH the enzyme is at its greatest effectiveness. Place this test tube in a boiling water bath for 5 minutes. Graph your results as ionic concentration x-axis vs.
Enter the time and distance the disk traveled in the column for Trial 1 in the data table below. Follow this link to get more helpful information about. You have hundreds of different enzymes in each of your cells. Compared with the untreated blended liver, did more, less or about the same amount of bubbles form? Add another 2 ml of hydrogen peroxide to the liver remaining in the first test tube. Enzymes are most often proteins and theirthree-dimensional shape is important to their catalytic activity. This experiment was conducted within a range of pH 3 to pH of 11, so this investigation is valid. The molarity of the chemicals used can also be put into question.
Each group will be assigned one or more specificexperiments in lab. So, some environments can make a liver enzyme effective, whereas others can prevent it from working at all. Submerge the graduatedcylinder to fill it with water. Now, feel the temperature of the test tube with your hand. The shape of the chart itself shows the decrease in needed reaction time, proving our analysis to be correct. Catalase works to speed up the decomposition of hydrogen peroxide into oxygen and water. A water bath that hasbeen warmed to 37C.
To begin, click on an activity title. Other than the table of results the graph is also evidence to my true hypothesis. How would you like to see your designs show up in toy stores? The procedure was controlled by using the same method for the enzymatic reactions in the investigation. For example, after setting up theapparatus as described below, you will then measure the amount ofO 2 collected in the graduated cylinder after one minute. The room temperature potato produced the most bubbles because catalase works best at a room temperature. Hypothesis: I forecast that the more concentrated the hydrogen peroxide is the higher the volume of Make sure you rinse your glassware carefully between procedures.