List any factors that you think may affect the rate of photosynthesis. It may take a few times to get the disks to sink. Conclusion: This section should summarize your overall findings and discuss any experimental errors or problems you encountered. Group 3 had to make a Sucrose solution which had sugar with a molecular weight of 232 and stir it in a beaker with 300ml of water. Photosynthesis Lab The rate of photosynthesis can be measured in two different ways: the disappearance of substrate or the amount of products produced. Misael Acosta Photosynthesis Lab Report Ms. Objective : To observe and investigate the cell structure under the light microscope.
Designing an experiment to test the rate of photosynthesis
According to our data, the solution of water + sodium bicarbonate has caused the spinach leaves to begin respiration of photosynthesize causing them to rise in the presence of carbon dioxide. One potential error within the experiment is releasing all the oxygen within the syringe that is filled with the spinach discs and soap solution. Record the number of disks floating at the end of each minute, swirl the disks to dislodge any that may get stuck to the beaker or to each other. The best amount of sodium bicarbonate baking soda is just enough to dust the bottom of the cup. Other than these there were few places for error in this lab. We poured the diluted solution in the cuvette and put into the spectrophotometer. They will need to use critical thinking to design the experiment and analyze the collected data.
This is shown when comparing the three different solutions,. Water may have been contaminated Leaving the Elodea plant in too long or too little Not cutting the Elodea plant correctly Conclusion: Hypothesis is declined. There are two types of microscope known as light microscope and electron microscope. Three drops of unboiled chloroplasts were placed in cuvette 2 and 3, and three drops of boiled chloroplasts were placed in cuvette 4. Materials: test tube, Elodea cuttings, sodium bicarbonate baking soda , beaker with water, lamp Part 1: Measurement of Photosynthesis There are various set-ups that can be used to measure the rate of photosynthesis, each relies on counting the oxygen produced during the reaction.
There are many specific light microscopes such as the bright-field, dark-field, phase contrast, fluorescence and stereoscopic dissecting. Background: This lab is used to test the effect the respiration rates in plants in animals and how it affects the level of carbon dioxide present in the water. Hypothesis After reading the lab instructions but before starting the lab record your best educated guess about what will happen in the experiment. It increases magnification, resolution, and contrast. Also, humans who are parents can also get counseling about many human inheritable diseases. You should see air coming out the sides of the disks.
The reason behind measuring the light transmittance is to calculate the rate of photosynthesis in the chloroplasts. If leaves are placed in an alternate source of carbon dioxide in the form of bicarbonate ions, then photosynthesis can occur. Photosynthesis can be measured by either the disappearance of substrate or the accumulation of product. This proves that we need chloroplasts to do photosynthesis. The teacher can now review what a hypothesis is and how to write a specific, testable hypothesis.
If the cup with bicarbonate solution allows the leaf disk to produce oxygen by the process of photosynthesis then the leaf disk in the bicarbonate solution will be the first to float within the 15 minutes allowed. The complex chemical reactions of photosynthesis and cellular respiration help meet the energy needs of living things. Place this set into a control beaker that contains only water and expose to light. The leaf becomes buoyant and floats. The graph looks as it was expected to look with.
Light reactions take in water and light to make oxygen. Then 4 test tubes were prepared. When there is more carbon dioxide in the surrounding environment, more carbon can be fixed and photosynthesis can occur faster. There is not much carbon dioxide in water, therefore photosynthesis occurs very slowly. Abstract For this lab the rate of photosynthesis was analyzed upon plant leaves.
Check to see if the plant disks sink in the water. The purpose of this experiment is to see how different concentrations of carbon dioxide affect the rate of photosynthesis in spinach leaves. Cuvette 3 does have a higher transmittance, however Cuvette 4 stays for the most part, the same and does not have an increase in percent transmittance. According to our results, the value of light went through the substance in test tube 1 was 1. What evidence supports those claims summarize data? Photosynthesis Experiment: Describe your experiment, include your hypothesis and data collected.
The foam produced is just water, soap, and oxygen so you can clean it up with a sponge and pour any extra liquid left in the bottle down the drain. This could mean that substrate below. The spectrophotometer may have not been calibrated correctly because this was the first time this particular one had been used. Use the text book or internet to label the following tissues; Upper epidermis, lower epidermis, palisade mesophyll, spongy mesophyll, vein label both xylem and phloem , guard cells, stoma 1 In which of the labeled structures, does most of the photosynthesis occur? Instead of carbon dioxide, what will be used to as the reactant in this lab? The results seem to follow what was thought to have happened. By increasing the use of carbon dioxide by plants, it may help slow global climate change. No, for a different solute there would be a different solvent rate. For animal cells, the process is known as cellular respiration by which cells break down complex molecules, such as sugars, to release carbon dioxide.
It was only due to time, not distance. Photoautotrophs are organisms that use photosynthesis to sustain themselves with nutrients Cavendish, 2004. We label the test tubes and pour each of the things that are necessary for our experiment. However, if the carbon dioxide and oxygen were removed the leaves would sink. This gives a green line across the paper, which contains the pigments of the leave. However the process to get this energy is different for plant and animal cells. The light energy absorbed is first transferred by exited electrons to reaction centers.
