Unknown bacteria. Isolation and Identification of Two Bacterial Unknowns (Theory) : Microbiology Virtual Lab II : Biotechnology and Biomedical Engineering : Amrita Vishwa Vidyapeetham Virtual Lab 2019-02-15
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Microbiology Unknown Lab Report
Words: 1675 - Pages: 7. However, surprisingly little has been published on the essential and beneficial roles of human intestinal bacteria. This condition is caused by the excess amount of nitrate that is then converted into nitrite by the digestive system. Indeed, the non-medical microbiology community has not only studied this symbiotic partnership in ruminants, as can be found in any textbook, but even in protozoa. Furthermore, the Unknown J did not produce or metabolize starch. A gram stain reaction test is used to differentiate between two bacterial species.
The following test is the coagulase test which shows if bacteria are capable of coagulating oxolated plasma. The name should be italicized or underlined. In healthcare, we are sure to use sterile technique when doing certain tasks as not to introduce infection to a patient. Starch Hydrolysis Test: Amylases are a class of enzymes that are capable of digesting these glycosidic linkages found in starches. Lipid Hydrolysis: Trybutyrene agar is used for the detection and enumeration of lipolytic microorganisms in food and other material Fig 9. Materials and Methods A mixed culture of two unknown bacteria was provided by the instructor. In order to know what group your bacteria falls into, you have several methods.
They were Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris. Some microorganisms possess an enzyme called gelatinase, which breaks down gelatin into amino acids. So, growth on each plate should favor only one of the unknowns. In order to do the streak method, an inoculating loop was sterilized with a Bunsen burner and put into the unknown specimen. This bacterium is also known by the names hay bacillus or grass bacillus 1.
Try to avoid unpasteurized dairy products. According to microbiology instructor Gram positive bacteria was Staphylococcus aureus, that leads to the conclusion that final Urea test had shown wrong results. Environmental and Industrial Use of Bacillus Subtilis. When find an unknown bacterium, you first make a pure culture of it on an agar plate. Some of these organisms have distinct qualities that set them apart from one another, such as the exposure to certain environments. Gram stains were performed on each culture.
Experiment for Identification of Unknown Bacteria (With Figure)
Using a wire loop, a small sample of the numbered test tube was plated on a nutrient agar using the quadrant streak method 4. Exercising care in sterile technique, so as not to contaminate cultures and thereby obtain spurious results, the bacteria is inoculated into respective media to carry out the different biochemical tests. Words: 7723 - Pages: 31. After the Gram stain was completed, the bacteria were streaked on a Mannitol-Salt Agar plate and a Catalase test was performed. Identification of bacteria is important because they are not only useful but potentially dangerous as well. After incubation, this nutrient agar had great results with many isolated colonies.
All those tests are done in laboratories, in hospitals, etc. Staining Reactions: Staining is a simple basic technique that is used to identify microorganisms. After successful isolation of both bacteria, there were no more issues encountered in identifying either. Scientific writing is written differently from other types of writing. .
It should be kept in mind that, results might vary depending on the strains of each species used and the length of time the bacteria has been maintained in stock culture. But even today, the popular view of micro-organisms is focused nearly exclusively on those that cause disease. Hence after the gram staining, the gram positive cells appear as purple and gram negative cells appear as pink Fig 2. This slant is tougher and denser than slant 37. The antiserum of particular bacteria is produced by injecting killed or attenuated cells of those bacteria say X into a laboratory animal.
The purpose of this study was to identify the unknown bacterium using biochemical tests and various methods that had been learned from previous the microbiology laboratory class. Gram negative family includes Shigella, Salmonella, Proteus, Klebsiella,Escherichia,Enterobacter etc. Therefore, when working with bacteria you must wear a lab coat, safety glasses and gloves. Firstly, the Gram positive unknown was rod shape. A fermentation test was done.
